Peanut allergies can trigger severe allergic reactions, including anaphylaxis, which can be life-threatening for allergic individuals even with trace amounts. Identifying peanut allergens is vital for maintaining food safety, legal compliance, and the welfare of consumers. Several crucial considerations, including strict labeling laws, legal liability, traceability and risk management, and preventing cross-contamination, stem from the significance of peanut allergen detection in today's food and other businesses. Beyond food industries, peanut-derived ingredients can be present in some cosmetics or personal care products and restaurants and food service to verify the absence of peanuts in products or menu items preventing accidental exposure for customers with allergies. Conventional techniques may depend on identifying peanut proteins, which may be vulnerable to cross-reactions with other legumes; however, RT-PCR targets particular DNA sequences specific to peanuts, eliminating false positives and guaranteeing precise detection of allergens related to peanuts. For customers with severe allergies, this specialized kit also ensures that allergens, even at low concentrations, are not overlooked, providing a higher standard of protection.